profinity exact cartridges  (Bio-Rad)

Journal: PLOS ONE

Article Title: Importance of annexin V N-terminus for 2D crystal formation and quick purification protocol of recombinant annexin V

doi: 10.1371/journal.pone.0278553

Figure Lengend Snippet: The purity of recombinant annexin V was analyzed by 15% (w/v) SDS-PAGE. M, molecular weight maker; Whole cells, whole proteins of the cell lysate; DEAE, annexin V protein after DEAE chromatography; Profinity eXact, annexin V protein after Profinity eXact purification. A band of ≈50 kDa was observed as a main band after DEAE chromatography, but after the tag was removed during elution procedure, a band consistent with the molecular weight of annexin V (36 kDa) was detected. The band position of annexin V after Profinity eXact purification was indicated by the arrow.

Article Snippet: The column was washed with 10 column volume of the same buffer, and the flow through fraction containing annexin V was applied to a Profinity eXact TM (exact affinity cleavage technology, Bio-rad, CA, US) protein purification column equilibrated with 100 mM NaPi (pH 7.2) in cold room (4°C).

Techniques: Recombinant, SDS Page, Molecular Weight, Chromatography, Purification